Figure 2.

Expression of PnCO in continuous darkness. Five-day-old light-grown seedlings were transferred to darkness at time 0. Cotyledons were harvested for RNA isolation at the times indicated. Poly(A)⁺ RNA preparations were analyzed by RNA gel blotting using a 720-bp cDNA of PnCO as probe. Residual 23S rRNA served as loading control. Plants returned to continuous light after 16 h of darkness or at later time points developed six or seven flower buds after 1 month in continuous light. Plants receiving 12 h of darkness or less remained vegetative.