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Expression of PnCO following night-break treatment. Five-day-old light-grown seedlings were transferred to darkness at time 0. Cotyledons were harvested for RNA isolation at the times indicated. The night break was given after 8 h of darkness. Poly(A)+ RNA preparations were analyzed by RNA gel blotting. The PnCO and CAB probes were as in Figure 2. 23S RNA served as loading control.
