Figure 5.

The unspliced long intron of the PnCO(li) transcript. ATG and TGA indicate the start and stop codons in PnCO(ni), which encodes the protein shown in Figure 1. In PnCO(li) the open reading frame is interrupted between nucleotides 888 and 1,181 by the intron sequence shown above. This introduces the premature stop codon TAA (highlighted). A second open reading frame of 429 bp starts at the highlighted ATG and ends at the same stop codon as does the uninterrupted PnCO(ni). The short intron sequence in PnCO(si) is underlined. It results in a stop codon (TGA) between nucleotides 1,228 and 1,230.