Figure 8.

RT-PCR analysis of PnCO expression in transgenic Arabidopsis plants. Poly(A)⁺ RNA preparations used for reverse transcription were isolated from aerial parts of plants harvested at the time when the first flower bud appeared in the rosette. Primers from the regions flanking the intron (TN178 and TN179, see Fig. 1) were used for the RT-PCR reaction. The PCR products were separated on a 2% (w/v) agarose gel. Lanes 1 and 2 are control reactions (without added reverse transcriptase) for the reactions in lanes 3 and 4, respectively. Lane 3, RT-PCR products from plants transformed with 35S::PnCO(li). Lane 4, RT-PCR product from plants transformed with 35S::PnCO(ni). Lane 5, Reference DNA amplified with the same primer set from the plasmids PnCO(ni), PnCO(si), and PnCO(li). The upper band 1 kb in length corresponds to DNA derived from PnCO(li); the lower band is about 700 bp in length and corresponds to PnCO(si) and PnCO(ni), which are 26 bp apart.