Table 3.
List of primers used to genotype the gene sax-7 when build strains
| Allele | Primer | Sequence | PCR product(s) (bp) | Cosmid coordinates |
|---|---|---|---|---|
| qv30 | Mutant specific | |||
| oCB747 | tctctcaaaattcttcgcaagc | 326 | C18F3 8252…8273, forward | |
| oCB1025 | cgggaagaaatgaaacagga | C18F3 28531…28550, reverse | ||
| Wild-type specific | ||||
| oCB1022 | tggtggtagcgatggtgtag | 609 | C18F3 12312…12331, forward | |
| oCB1023 | agttcgatgttctcggctgt | C18F3 12901…12920, reverse | ||
| qv25 | oCB1022 | tggtggtagcgatggtgtag | 656 (mt), | C18F3 12312…12331, forward |
| oCB1023 | agttcgatgttctcggctgt | 609 (wt) | C18F3 12901…12920, reverse | |
| qv26 | oCB1022 | tggtggtagcgatggtgtag | 645 (mt), | C18F3 12312…12331, forward |
| oCB1023 | agttcgatgttctcggctgt | 609 (wt) | C18F3 12901…12920, reverse | |
| nj48 | oCB1022 | tggtggtagcgatggtgtag | 257 (mt), | C18F3 12312…12331, forward |
| oCB208 | gagttattggggtattttagcg | 825 (wt) | C18F3 13115…13136, reverse |