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. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Obesity (Silver Spring). 2021 Jul 5;29(8):1309–1319. doi: 10.1002/oby.23199

Figure 1: SCO promotes adipogenesis in the absence of MIX, 1-methyl-3-isobutylxanthine.

Figure 1:

(A) Schematic of study design. 3T3-L1 preadipocytes were treated with full differentiation cocktail (MIX (M), DEX (D) and insulin (I); MDI), MD, DI or D with either vehicle (DMSO), SCO or ROSI for three days. Following the third day, media was changed to include only insulin for all conditions for up to two days. (B) Lipid was detected using Oil Red O staining and (C) lipid quantification was performed by measuring the absorbance of staining following an isoproterenol extraction method. This experiment is a representative of an experiment independently performed four times on different batches of cells. Data are normalized to standard differentiation (DMSO MDI condition) and presented as average ± SD. Data are normalized to standard differentiation (DMSO MDI condition) and presented as mean ± SD. Statistical analyses were performed using a one-way ANOVA with multiple comparisons, significance was set at p<0.05. * indicates significance compared to DMSO; $ indicates significance between SCO and ROSI.