Figure 3.

Oligodendrocyte maturation genes also dysregulated in SCA3 mouse cerebellar tissue. A–E, Validation by qPCR of gene expression changes over time in SCA3 cerebellum corresponding to oligodendrocyte genes across developmental stages. Validated genes include Smoc1, Ugt8a, Plp1, Mobp, and Mal. Data (mean ± SEM) are normalized to Q84/Q84 samples (n = 4-6 per condition). Unpaired parametric t tests, assuming equal SD, were performed: *p < 0.05; **p < 0.01. F, G, Representative Western blot image and quantification of MOBP, a mature oligodendrocyte marker, in cerebellar tissue at 8 and 16 weeks of age (n = 3 or 4 mice per genotype) analyzed by one-way ANOVA with Tukey's multiple comparisons test: *p < 0.05. H, qPCR and (I) Western blot analyses of oligodendrocyte maturation markers at 16 weeks show no changes in the cerebellum of ATXN3 KO mice by one-way ANOVA with Tukey's multiple comparisons test.