Figure 3.

Correlation of heme- and ferrous ion-induced polyreactivity of clinical-stage Abs. The graph depicts the correlation (Spearman analyses) of the fold increase in the antigen-binding intensity (averaged gain in reactivity for three unrelated proteins) after treatment of Abs with heme or ferrous ions. The reactivity of each Ab is presented as an individual circle. The correlation was characterized by Spearman coefficient ρ = 0.38 and p < 0.0001. The data for heme-treated Abs were taken from a previously published study [23].