Figure 4.

Effects of light on LeDI-2 expression and shikonin production in hairy root cultures of L. erythrorhizon in M9 medium. A, Left panels show the accumulation of LeDI-2 mRNA in hairy roots cultured in the dark, and right ones are those under illumination. B, Cell growth and accumulation of shikonin in hairy roots cultured in the dark (left) or under illumination (right). The culture was started by inoculating 0.5 g of hairy roots that had been cultured in Murashige and Skoog liquid medium in the dark. C, Light-induced up- and down-regulation of LeDI-2 expression during the culture period. Hairy root cultures were transferred from a dark to light (left) or light- to-dark (right) condition 7 d after inoculation.