Figure 8.
A, Biosynthetic pathway of shikonin. When the expression of geranyltransferase is inhibited by light, the excess amount of PHB is accumulated as its glucoside form. PHB-OG, O-glucoside of PHB; GPP, geranylpyrophosphate. Shikonin production and PHB:geranyltransferase activity in control and LeDI-antisense hairy root clones are indicated in B and C, respectively. The accumulation level of PHB-OG in the root tissues of those clones are also monitored in D. Cells were cultured in the dark or under continuous light (80 μE/m−2 s−1) with fluorescent lamps.