a-c) CFBE cells cultured under high glucose show significant increases in mRNA expression levels of MMP-9 (a), COX-2 (b), and RAGE (c) compared to CFBE cells cultured under normal glucose conditions (n=7 CF lungs). d) CFBE cells cultured under high glucose show a significant increase in expression levels of IL-1β protein in the basolateral media as determined by ELISA (n=18, triplicates from 6 CF lungs) and so was (e) IL-8 (n=24, triplicates from 6 CF lungs). Expression levels of IL-6 (f) are not significantly different in the basolateral media of CFBE cells cultured under normal vs. high glucose (n=18, triplicates from 6 CF lungs). * p < 0.05, n.s.=not significant, Wilcoxon test. Data are shown as mean ± S.E.M.