Extended Data Table 1.
Cryo-EM data collection, refinement and validation statistics.
| MFSd2a (EMDB-24252) (PDB 7N98) |
|
|---|---|
| Data collection and processing | |
| Magnification | 105,000 |
| Voltage (kV) | 300 |
| Electron exposure (e-/Å2) | 53 |
| Defocus range (μm) | 1.2-2.5 |
| Pixel size (Å) | 0.86 |
| Symmetry imposed | CI |
| Initial particle images (no.) | 2,471,140 |
| Final particle images (no.) | 90,577 |
| Map resolution (Å) | 3.50 |
| FSC threshold | 0.143 |
| Map resolution range (Å) | 11.0-2.7 |
| Refinement | |
| Initial model used (PDB code) | 4M64 |
| Model resolution (Å) | 3.98 |
| FSC threshold | 0.5 |
| Model resolution range (Å) | 200-3.35 |
| Map sharpening B factor (Å2) | −184 |
| Model composition | |
| Non-hydrogen atoms | 3626 |
| Protein residues | 473 |
| Ligands | 0 |
| B factors (Å2) | |
| Protein | 139.58 |
| Ligand | - |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.004 |
| Bond angles (°) | 0.716 |
| Validation | |
| MolProbity score | 2.08 |
| Clashscore | 11.62 |
| Poor rotamcrs (%) | 0.27 |
| Ramachandran plot | |
| Favored (%) | 91.5 |
| Allowed (%) | 8.5 |
| Disallowed (%) | 0 |