Figure 10.

Dependence of R-induced protoplast swelling on Ca²⁺ in the bathing medium. The experiment was conducted as described for Figure 7. Protoplasts isolated from peeled stems of R-grown pea cv Alaska seedlings were washed with and suspended in a Ca²⁺-free medium (see Fig. 9). The protoplasts were incubated under R on the microscope stage and the data from 0 to 20 min were obtained. Protoplasts were washed with and resuspended in the standard medium and the data after 45 min were obtained. The means ± se from 20 or 21 protoplasts are shown.