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The changes in protoplast volume during prolonged R irradiation after pretreatment with FR and darkness. Protoplasts isolated from peeled stems were pretreated with a 3-min FR pulse and darkness as illustrated and were subsequently incubated under background R. The experimental conditions were identical to those used to obtain the data in Figure 3B except that the volume was monitored for a longer period. The means ± se from 26 protoplasts are shown.
