Figure 8.

The effect of vanadate on R-induced protoplast swelling. Protoplasts isolated from peeled stems of R-grown pea cv Alaska seedlings were washed with and suspended in the standard medium (A) or the medium to which vanadate was added at a concentration of 500 μm (B). The protoplasts were incubated under R as described for Figure 1. Time zero corresponded to 15 min after the onset of incubation (about 40 min after the protoplasts were put in contact with vanadate). The vanadate-treated protoplasts (A) retained a nearly constant volume during the additional 46 min. The means ± se from 20 (A) or 22 (B) protoplasts are shown.