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. 2022 Feb 28;18(3):e10798. doi: 10.15252/msb.202110798

Figure 1. TIMS enables virtually noise‐free spectra and ultra‐high sensitivity proteomics.

Figure 1

  • A, B
    The TIMS‐qTOF principle separating singly charged background peaks from multiply charged peptide precursor ions, making precursor ions visible at extremely low signal levels (0.8 ng HeLa digest).
  • C
    Quantified proteins from a HeLa digest dilution series from 25 ng peptide material down to 0.8 ng (arrow), roughly corresponding to the protein amount contained in three HeLa cells on our initial LC–MS setup (See Material and Methods).
  • D
    Linear quantitative response curve of the HeLa digest experiment in C (Box and Whiskers; The middle represents the median, the top and the bottom of the box represent the upper and lower quartile values of the data, and the whiskers represent the maximum and minimum value of the data).
  • E
    Quantitative reproducibility of two successive HeLa digest experiments at the lowest dilution (technical LC–MS/MS replicates).