Figure 3.

Pedigree of T. pusilla families. The parental generation (F₀) comprised the field-grown plants from which TA136 seeds were collected. Generation F₁ were plants germinated from TA136 seed and assayed in vitro for DMBQ responsiveness. After screening, selected plants were rescued from the assay plates and grown to maturity in soil; these individuals were given the pedigree numbers TP-2207 to TP-2214. Seeds were harvested from individual, self-pollinated plants. This second generation (F₂) was similarly screened for responsiveness to DMBQ, transplanted to soil, and self-pollinated. The F₃ generation was assayed in the same way. Pedigree numbers that follow a solid line represent individual plants. The numbers in parentheses that follow a dashed line represent the number of siblings that were analyzed, but not subsequently rescued.