Figure 1.

MYb11 and MYb115 affect reproductive timing, and MYb11 affects fertility and lifespan. (A) Daily brood size (hatched offspring) of C. elegans exposed to the two natural microbiota isolates, P. lurida MYb11 and P. fluorescens MYb115, compared to worms exposed to the food bacterium E. coli OP50. Hatched offspring were counted every day until the worms did not produce any more offspring for two consecutive days. Presented here are pooled data combined from 5 independent experiments (n= 75 (OP50), n=69 (MYb11), n=43(MYb115), also see Supplementary Table ). “x” indicates significantly higher hatched offspring for worms on the natural microbiota isolates, while “o” indicates significantly higher numbers for worms on OP50. *p < 0.05, as determined by Kruskal-Wallis test, with Wilcoxon pairwise comparison and FDR correction. (B) Lifespan analysis of C. elegans N2 under different bacterial conditions on PFM plates. Alive, dead, and missing worms were counted until all worms were dead, i.e., failed to respond to the light touch of a platinum wire picker. Shown are pooled data combined from four independent experiments (n=573 (OP50), n=459 (MYb11), n=554 (MYb115), also see Supplementary Table ). P-values were determined with Kaplan-Meier analysis and log-rank test, and are considered significant according to: *p < 0.05. Horizontal ticks represent censored data (missing worms).