Figure 4.

miR-338-3p blocks TGFβ-induced matrix proliferation. Primary human lung fibroblasts from three different donors were treated with 200 nM nontargeting control microRNA or miR-338-3p ±1 ng/mL TGFβ. A: 72 h after treatment cells were counted utilizing trypan blue, only live cells were counted, n = 4/group. B: ATP production was assessed by cleavage of a luminescent substrate 72 h after treatment, n = 8/group. Cell death was assessed via LDH release prior to treatment (C) and 72 h after treatment (D), n = 5/group (***P < 0.005, ****P < 0.0001 by ANOVA).