Table 1. Permutation testing of changes in clonality after transplantation of erythropoietin (EPO)-exposed hematopoietic stem and progenitor cells (HSPCs).

Same data as in Figures 1—4,7. HSPCs or multipotent progenitor 2 (MPP2) were cultured with different concentrations of EPO (160 ng/ml or 1000 ng/ml) for 16 hr, and when indicated a soluble dose of EPO (133 µg/kg) was injected together with barcoded HSPCs at the moment of transplantation. Barcodes in the erythroid (E), myeloid (M), B-lymphoid (B) lineage, dendritic cell (DC), and HSPCs were analyzed 4 weeks after transplantation and categorized by bias using a 10% threshold. For the data of Figures 1, 2 and 7, the output of MB and ME classified barcodes to the B, M, and E lineages was analyzed using a permutation test. For the data of Figure 3, the output of MBE and MB classified barcodes to the DC lineage was analyzed. For the data of Figure 4, the output of barcodes present in HSPCs to the B, M, and E lineages was analyzed using a permutation test. By permutating the mice of the control and EPO groups, the random distribution of this output was generated and compared to the real output difference between the control and EPO groups. A p-value was generated as in Tak et al., 2019.

Figure	Condition	p-Value
		MB in B	MB in M	ME in E	ME in M
Figure 1	HSPCs 160 ng/ml	0.02	0.04	0.02	0.04
Figures 1 and 2	HSPCs 1000 ng/ml	0.0075	0.0071	0.0071	0.012
Figure 2	HSPCs 160 ng/ml + inj.	0.01	0.016	0.012	0.011
Figure 2	HSPCs 1000 ng/ml + inj.	0.0018	0.0018	0.002	0.0025
Figure 7	MPP2 1000 ng/ml	0.006	0.008	0.004	0.004
		MBE in DC	MB in DC
Figure 3	HSPCs 1000 ng/ml	0.07	0.0075
		HSPC in B	HSPC in M	HSPC in E
Figure 4	HSPCs 160 ng/ml	0.035	0.029	0.029
	HSPCs 1000 ng/ml	0.008	0.06	0.01
		ME in E	ME in M
Figure 8	HSPCs 160 ng/ml	0.016	0.0025
	HSPCs 1000 ng/ml	0.00625	0.0038