Figure 3.

Overview of AAV vector design. (A) Schematic representation of AAV structure. AAV virions are non-enveloped 25 nm icosahedral particles. (B) Outline of wild type AAV genome and AAV vector plasmid with the transgene expression cassette. AAV vector particles are assembled in adenoviral E1-expressing HEK 293 cell line, which is co-transfected with transgene AAV vector plasmid, a helper plasmid and a rep/cap plasmid. Alternatively, AAV vectors can be produced in insect cells, which are co-infected with ITR-flanked transgene and rep/cap recombinant baculoviruses.

ITR = inverted terminal repeat; pDNA = plasmid DNA; ssDNA = single-stranded DNA