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. 2022 Mar 1;132(5):e144904. doi: 10.1172/JCI144904

Figure 4. Coding variants in the BAR domain compromise PICK1’s function in insulin storage in INS-1E cells.

Figure 4

(A) Representative confocal images of INS-1E cells transduced with lentiviral constructs reexpressing PICK1 with each of the 4 coding variants and immunostained for GFP (yellow), PICK1 (cyan), and insulin (magenta). The merged images show PICK1 and insulin immunosignals. Examples of transduced cells (GFP-positive) are outlined with yellow dotted lines, and untransduced cells are outlined with white dotted lines. Scale bar: 10 μm. (B and C) Quantification of PICK1 and insulin immunosignals from A. The dashed lines show values for KD and KD + WT PICK1 and insulin immunosignals (mean ± SEM) from Figure 3, B and C. Data are shown as mean ± SEM. **P < 0.01, ***P < 0.001, KD + R158Q (n = 112), KD + R185Q (n = 50), KD + R197Q (n = 64), KD + R247H (n = 102) compared with KD + WT (n = 108); Kruskal-Wallis test followed by Dunn’s multiple-comparison test.