Skip to main content
. 2022 Mar 1;132(5):e141797. doi: 10.1172/JCI141797

Figure 3. SIRPγ serves as a negative upstream regulator of the MST1/LATS1 axis to promote YAP activation and cancer organoid growth.

Figure 3

(A and B) Immunoblotting analysis of indicated proteins in control and SIRPγ-knockdown A549 (A) and H1975 (B) cells. (C and D) Immunoblotting analysis of indicated proteins in control and SIRPγ-overexpressing cells. (E) Immunoblotting analysis of indicated proteins in SIRPγlo/– and SIRPγhi A549 cancer cells. (F and G) Stem cell sphere assay of YAP-overexpressing or -knockdown A549 (F) or H1975 (G) cells (1 × 103 cells/well). (H and I) Stem cell sphere formation assay and immunoblotting analysis of vector- and SIRPγ-overexpressing A549 (H) or H1975 (I) cells with or without YAP knockdown. (J) H&E staining of tumor tissue derived from patients with LUAD. Scale bars: 50 μm. (K and L) Representative images are shown for the growth of LUAD-derived organoids of indicated groups grown in matrigel-supplemented media for 7 days. Quantification of the growth of organoids. Scale bars: 20 μm. All experiments were carried out at least in triplicate and the data are presented as the mean ± SD. *P < 0.05; **P < 0.01 by paired, 2-tailed Student’s t test (F and G, left panels) or 1-way ANOVA (F, G [right panels in both], H, I, K, and L).