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. 2022 Mar 1;132(5):e141797. doi: 10.1172/JCI141797

Figure 4. SIRPγ recruits PP2A to dephosphorylate MST1 and promotes YAP signaling activation.

Figure 4

(A and B) Immunoprecipitation analysis of the interaction between SIRPγ, PP2A, and MST1. PP2Ac is the catalytic subunit of PP2A. IB, immunoblot; WCE, whole-cell extract. (C) Immunoblotting analysis of indicated proteins in control and PP2A-knockdown cells. (D) Immunoblotting analysis of indicated proteins in vector- and SIRPγ-overexpressing cells with or without PP2Ac knockdown in A549 cells. (E and F) Immunoprecipitation analysis of the interaction between PP2A and MST1 in control and SIRPγ-knockdown A549 cells. (G) Immunoprecipitation analysis of the interaction between SIRPγ, PP2A, and MST1 in vector-, SIRPγ-, SIRPγ C terminus–, or SIRPγ C terminus and transmembrane domain (C&M) deletion mutant–overexpressing A549 cells.