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. 2022 Mar 1;132(5):e141797. doi: 10.1172/JCI141797

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© 2022 Xu et al.

This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) qRT-PCR analysis of indicated genes in control and SIRPγ-knockdown A549 cells. (B) Immunoblotting analysis of indicated proteins in control and SIRPγ-knockdown A549 cells. (C) Flow cytometric analysis of CD47 in control and SIRPγ-knockdown A549 cells. (D) qRT-PCR analysis of SIRPG in SIRPγ^hi and SIRPγ^lo/– A549 cells. (E) qRT-PCR analysis of indicated genes in SIRPγ^hi and SIRPγ^lo/– A549 cells. (F) Flow cytometric analysis of CD47 protein expression in SIRPγ^lo/– and SIRPγ^hi A549 cells. (H) qRT-PCR analysis of indicated genes in vector- and SIRPγ-overexpressing A549 cells. (G) Immunoblotting analysis of indicated proteins in vector- and SIRPγ-overexpressing A549 cells. (I) Left: Schematic diagram of vector- (V, control) and SIRPγ-overexpressing (OE) A549 cell coculture. Right: Immunoblotting analysis of indicated proteins in control cells cocultured with control cells and control cells cocultured with SIRPγ-overexpressing cells. All experiments were carried out at least in triplicate and the data are presented as the mean ± SD. **P < 0.01 by paired, 2-tailed Student’s t test (A, D–F, and H) or 1-way ANOVA (C).