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. 2022 Mar 1;132(5):e141797. doi: 10.1172/JCI141797

Figure 6. SIRPγ promotes IL-1β and GM-CSF release to sustain CD47 expression through YAP signaling.

Figure 6

(A and B) ELISA analysis of indicated cytokines in control and SIRPγ-knockdown A549 cells. (C and D) ELISA analysis of indicated cytokines in vector- and SIRPγ-overexpressing A549 cells. (E and F) ELISA analysis of indicated cytokines in vector- and SIRPγ-overexpressing cells with or without YAP knockdown. (G) qRT-PCR analysis of CD47 expression in control and SIRPγ-knockdown cells with or without IL-1β (100 ng/mL) and GM-CSF (30 ng/mL) treatment for 24 hours. (H) Flow cytometric analysis of CD47 expression in control and SIRPγ-knockdown cells with or without IL-1β (100 ng/mL) and GM-CSF (30 ng/mL) treatment for 24 hours. (I) Flow cytometric analysis of CD47 protein expression in vector- and SIRPγ-overexpressing A549 cells with or without YAP knockdown. (J) qRT-PCR analysis of indicated genes in control and SIRPγ-knockdown cells with or without YAP overexpression. All experiments were carried out at least in triplicate and the data are presented as the mean ± SD. *P < 0.05; **P < 0.01 by paired, 2-tailed Student’s t test (A–D) or 1-way ANOVA (E–J).