Figure 4. High dose of AAV9/MFSD8 GT tends to ameliorate SCMAS accumulation in the brain and spinal cord of KO mice.

High (5 × 10¹¹ vg/mouse) or low (1.25 × 10¹¹ vg/mouse) dose of AAV9/MFSD8 vector was administered intrathecally to equal numbers of male and female mice at P7–P10. At 4.5 months of age, mouse brain and spinal cord were harvested for IHC staining to detect SCMAS (A). Histology images with 1 section/animal were digitized with a ScanScope slide scanner and analyzed using custom analysis settings in the HALO Image Analysis Platform. Results are presented as the percentage of area staining positive for SCMAS by tissue region (B). A ROUT test was used first to remove any outlier. Each data point represents a measurement from an individual animal (n = 5–6), with lines representing the mean measurement ± SEM. Percentages above the KO-High data points indicate the reduction in the mean compared with KO-Veh. Data sets that passed tests for normality or homogeneity of variance were analyzed using 1-way ANOVA with α set at 0.05 and Dunnett’s correction for relevant pairwise comparisons. Data sets that did not pass tests for normality or homogeneity of variance were analyzed using the Kruskal-Wallis test with α set at 0.05 and Dunn’s correction for relevant pairwise comparisons. *P < 0.05; **P < 0.01; ***P < 0.001, compared with KO-Veh. Scale bars: 300 μm (cortex); 500 μm (hippocampus, cerebellum, spinal cord).