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. 2022 Mar 1;132(5):e142338. doi: 10.1172/JCI142338

Figure 2. Enhanced aortic γ-secretase levels with elastin deficiency.

Figure 2

(A and C) Lysates were analyzed by Western blotting for γ-secretase subunits NCT, PSEN1, PSEN2, and PEN2 and for GAPDH. In A, haSMCs were pretreated with Scr or siELN RNA, and in C, aortas of 2 WT or Eln–/– mice at P0.5 were pooled. (B and D) Densitometry of protein bands in A and C relative to GAPDH and normalized to Scr in B or WT in D (n = 3). *P < 0.05; **P < 0.01 by Student’s t test vs. Scr (B) or WT (D). Data are averages ± SD. (E and F) Ascending aortic cross sections of WT and Eln–/– mice at P0.5 stained for α-smooth muscle actin (SMA, marker of SMCs), nuclei (DAPI), and either PSEN1 in E or PSEN2 in F. n = 3 mice. Lu, lumen. Scale bars: 25 μm.