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. 2022 Mar 1;132(5):e142338. doi: 10.1172/JCI142338

Figure 6. On the Eln–/– background, Notch3 deletion attenuates hypermuscularization and stenosis and reduces SMC proliferation.

Figure 6

(A) Transverse sections of the ascending aorta from pups at P0.5 of indicated genotype were stained for SMA (SMC marker) and CD31 (EC marker). Lu, lumen. Scale bar: 100 μm. (B–D) Histograms represent medial thickness (B), lumen area (C), and medial wall area (D) of ascending aortas from A. n = 4 to 5 mice. **P < 0.01; ***P < 0.001; ****P < 0.0001 by 1-way ANOVA with Tukey’s post hoc test. (E) EdU was injected in pregnant dams at E18.5, and 8 hours later, embryos of indicated genotypes were collected. Cryosections of ascending aortas were stained for EdU, SMA, CD31, and nuclei (DAPI). Proliferative SMCs were marked by EdU+SMA+DAPI+ cells. Lu, lumen. Scale bar: 25 μm. (F) Histogram represents the percentage of SMCs that are proliferative in E. n = 3 mice. **P < 0.01 by 1-way ANOVA with Tukey’s post hoc test. All data are averages ± SD.