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. 2022 Mar 1;132(5):e157500. doi: 10.1172/JCI157500

Figure 6. Assessing amounts of GPIHBP1, relative to CD31, along the capillary lumen in tissue sections as well as the amounts of GPIHBP1, relative to CD31, in whole tissue extracts.

Figure 6

(A) Representative images of tissue sections that show the binding of an IRDye 800–labeled GPIHBP1 antibody (11A12; red) and an IRDye 680–labeled CD31 antibody (2H8; green) to the luminal surface of blood vessels in brown adipose tissue (BAT), heart, and lung of Gpihbp1+/+, Gpihbp1+/–, and Gpihbp1S/S mice. GPI, GPIHBP1. (B) Representative Western blots (with antibodies 11A12 and 2H8) of BAT, heart, and lung extracts from Gpihbp1+/+, Gpihbp1+/–, Gpihbp1S/S, and Gpihbp1–/– mice. (C) GPIHBP1/CD31 ratios (calculated from IRDye 800 and IRDye 680 signals) in sections of BAT, heart, and lung from Gpihbp1+/+ (n = 4), Gpihbp1+/– (n = 3), and Gpihbp1S/S (n = 4) mice. Ten sections were analyzed in each tissue of each mouse. (D) Relative amounts of GPIHBP1 and CD31 in BAT, heart, and lung extracts from Gpihbp1+/+, Gpihbp1+/–, and Gpihbp1S/S mice, as judged by quantification of Western blot bands (n = 4 independent experiments). NS, not significant; *P < 0.05; **P < 0.01; ***P < 0.001. Means were compared with 1-way ANOVA tests in panels C and D.