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. 2022 Feb 21;2022:8305271. doi: 10.1155/2022/8305271

Figure 4.

Figure 4

Antioxidant activity of CME of S japonica and its fractions. (a) DPPH radical scavenging activity. IC50 (µg/mL): CAT, 3.03 ± 0.15; CME, 24.12 ± 1.00; CHF, 5.01 ± 0.16; EAF, 19.10 ± 0.73; AQF, 44.57 ± 1.90; PEF, 48.48 ± 3.47. (b) Hydroxyl radical scavenging activity. IC50 (µg/mL): CAT, 9.00 ± 0.34; CME, 97.24 ± 1.09; CHF, 17.12 ± 0.23; EAF, 56.67 ± 0.63; AQF, 86.08 ± 1.88; PEF, 162.00 ± 1.76. (c) Ferric reducing power. At 100 µg/mL concentration, the absorbances are as follows: CAT, 2.539 ± 0.045; CME, 1.471 ± 0.027; CHF, 2.489 ± 0.039; EAF, 1.548 ± 0.033; AQF, 0.627 ± 0.020; PEF, 0.609 ± 0.014. (d) Total antioxidant capacity. At 100 µg/mL concentration, the absorbances are as follows: CAT, 2.147 ± 0.042; CME, 0.948 ± 0.022; CHF, 1.852 ± 0.035; EAF, 0.862 ± 0.018; AQF, 0.253 ± 0.016; PEF, 0.473 ± 0.016. (e) Lipid peroxidation inhibition. IC50 (µg/mL): CAT, 18.23 ± 0.54; CME, 92.91 ± 2.35; CHF, 33.42 ± 0.45; EAF, 137.70 ± 1.31; AQF, 157.63 ± 3.15; PEF, 248.20 ± 2.50. Catechin (CAT) was used as a reference standard. Results are expressed as mean ± SD (n = 3). Means with different letters (a–f) differ significantly (P < 0.05). CAT, catechin; CME, crude methanolic extract; CHF, chloroform fraction; EAF, ethylacetate fraction; AQF, aqueous fraction; PEF, petroleum ether fraction.