Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jan 19;18(3):342–351. doi: 10.1038/s41589-021-00965-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a–d, sACE2 carrying the v2.4 mutations has increased S binding compared with WT sACE2. Human Expi293F cells expressing myc-tagged S from 4 SARS-CoV-2 variants (Wuhan (a), B.1.1.7/Alpha (b), B1.351/Beta (c) and P.1/Gamma (d)) were incubated with monomeric sACE2-8 h (black) or dimeric sACE2₂-IgG1 (gray); bound protein was detected by flow cytometry. WT ACE2 proteins are shown as broken lines, v2.4 proteins are shown as solid lines. n = 3 independent replicates; data are shown as the mean ± s.e.m. e–i, Binding of sACE2₂.v2.4-IgG1 is comparable to clinically effective mAbs. Binding of mAbs versus sACE2₂.v2.4-IgG1 to the S proteins of SARS-CoV-2 VOCs (B.1.1.7/Alpha (e), B1.351/Beta (f), B.1.617.2/Delta (g) and P.1/Gamma (h)) and S protein of SARS-CoV-1 (i), as measured by flow cytometry. n = 3 independent replicates; data are shown as the mean ± s.e.m.

Source data