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. 2021 Dec 23;18(3):264–271. doi: 10.1038/s41589-021-00918-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 10 — a-d, Dose-response curves of β-arrestin recruitment (upper), endocytosis (middle) and G_i activation (bottom) on CCR1(wild-type) (a), CCR1(Y291^7.43A) (b), CCR1(T86^2.56A/W90^2.60A) (c) and CCR1(Y113^3.32F/Y255^6.51F) (d) induced by CCL15^L and CCL15^S [CCL15(30-92)]. N= three independent experiments for the endocytosis on CCR1(Y291^7.43A) and CCR1(Y113^3.32F/Y255^6.51F), performed with single replicates. N= four independent experiments for the others, performed with single replicates. e, Biased factors of CCL15^S [CCL15(30-92)] on wild-type and mutational CCR1s relative to CCL15^L. N= four independent experiments, performed with single replicates. The asterisk symbols indicated statistically significant difference (P =0.0072, P =0.0004, and P=0.0034 from left to right, **P <0.01) for mutants vs. wild-type CCR1 as determined by one-way ANOVA. f, The proportion of membrane to total expression of receptors. N= three independent experiments, performed with single replicates. P values were calculated using two-tailed Student’s t-test. The asterisk symbols indicated statistically significant difference (P <0.001, and P=0.9789 from left to right, ****P <0.0001) for mutants vs. wild-type CCR1. In a-f, all data were shown as mean±SEM.

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