Fig. 2. Cryo-EM structures of CCR1–G_i complexes in the CCL15^L-, CCL15^M- bound and apo states.

a,c,e, Cryo-EM density maps of CCR1-G_i complexes bound to CCL15^L (a), CCL15^M(c) and in the apo state (e). b,d,f, Models of CCR1–G_i complexes in three states (left). Sandy brown, Gα_i; purple, Gβ; sky blue, Gγ; gray, scFv16 and green, CCL15^L-bound CCR1 (b); brown, CCL15^L (b); dark khaki, CCL15^M-bound CCR1 (d); orange, CCL15^M (d) and plum, apo-state CCR1. Cryo-EM maps and atomic resolution models showing the binding pockets in the 7TM domains of two CCR1–G_i complexes (right). Atomic models and EM density shown as sticks and surfaces, respectively. EM density of CCL15^L and CCL15^M shown as a mesh.