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Immunoblot analysis of stacked spinach thylakoid membranes isolated after illumination with photosystem I-specific far-red light (>715 nm at 20°C for 130 min) in the presence of externally added photosystem I electron donors. The blot was incubated with antibodies directed against the holo-photosystem I complex isolated from barley. Each sample applied contained 1.0 μg of chlorophyll. 1, 0 control. 2 + 3, 130 min of illumination. 4 + 5, 130 min of darkness.
