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. 2022 Feb 28;8:89. doi: 10.1038/s41420-022-00885-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A The identification of neurons was conducted by immunofluorescence. DAPI (blue), NeuN (green), 200×, scale bars = 100 μm. B Relative expression of TUG1 (n = 5). C The transfection efficiency of TUG1 (n = 5). D MTT assay (n = 6). E Relative protein levels of COX2, Bcl-2 and Bax in neurons (n = 5). Relative expression of miR-204-5p (F) and COX2 mRNA (G) in neurons (n = 5). Data are presented as the mean ± SD. **p < 0.01, ***p < 0.001.