Fig. 5. A sub-population of microglia from SOD1G93A mice exhibits increased immune response pathways at peri-symptomatic onset stage of disease.
a Volcano plot of DEGs between genotypes in microglia. Genes with FDR < 0.1 are colored red (up-regulated) or blue (down-regulated). b Microglia KEGG gene sets that are significantly differentially expressed between genotypes in both ROAST and CAMERA displayed as a network. c UMAP plot of microglia sub-clusters. d Bar chart summarizing the number of DEGs in each sub-cluster (FDR < 0.1). Positive and negative y-axis values indicate number of up-regulated and down-regulated genes, respectively. Sub-cluster 0 microglia KEGG (e) and Reactome (f) gene sets that are significantly differentially expressed between genotypes in both ROAST and CAMERA displayed as a network. For b, e, and f: Internode line thickness reflects number of shared genes. Node size represents number of genes in the set. Red nodes denote up-regulation. g Density plot of EGR1 regulon activity scores for microglia sub-cluster 0. N = 3 independent mice per genotype per sex.