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. 2022 Feb 15;13:833040. doi: 10.3389/fimmu.2022.833040

Figure 2.

Figure 2

Effect of P.M OMVs on osteoclast genes, proteins, and function. (A) Effect of P.M OMVs (0.15 and 0.3 μg/ml) and LPS (5 and 10 ng/ml) on Jdp2 gene and (B) protein expression. Differentiation of (C) naive and (D) inflammatory OCs after treatment with P.M OMVs (0.15 and 0.3 μg/ml) or P.M LPS (5 and 10 ng/ml). P.M OMVs inhibited the expression of (E) OC-related (Acp5, MMP9, CTSK, NFATc1, c-Fos, and itgβ3) genes, (F) actin ring formation, (G) expression of proteins (MMP9, CTSK, NFATc1, c-Fos, and IkBα) and (H) bone resorption (n = 6). n indicates number of mice. P.M, Proteus mirabilis. Scale bar 200 μm in (C, D, H) and 50 μm in (F). Data are from 3 independent experiments and represented as mean ± SD. ##p < 0.01; ###p < 0.001; ####p < 0.0001 compared to M-CSF; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 compared to M-CSF + RANKL. ns, not significant.