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. 2022 Feb 15;13:833040. doi: 10.3389/fimmu.2022.833040

Figure 5.

Figure 5

Effect of mmu-miR-96-5p on OC differentiation and apoptosis. (A) Effect of mmu-miR-96-5p mimic or inhibitor on OC formation. (B, C) Osteoclast apoptosis, (D, E) ROS production, and (F, G) mitochondrial membrane potential level after miR-96-5p mimic or inhibitor treatment. (H) Western blot analysis of caspase-3, Bcl-2, and Bax. (I) Cyto c expression in the cytoplasm and mitochondria with or without P.M OMV treatment. P.M, Proteus mirabilis. Scale bar = 200 μm. Data are from 3 independent experiments and given as mean ± SD. #p < 0.05 compared to NC mimic. ****p < 0.0001 compared to M-CSF+RANKL. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 compared to the NC inhibitor. ns, not significant.