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. 2022 Feb 15;13:833040. doi: 10.3389/fimmu.2022.833040

Figure 6.

Figure 6

Effect of P.M OMVs on miR96/Abca1 mediated mitochondria-dependent apoptosis. (A) Osteoclast apoptosis, (B) ROS production, (C) Western blot analysis of caspase-3, Bcl-2, and Bax, (D) mitochondrial membrane potential level, and (E) Cyto c expression in the cytoplasm and mitochondria after mimic transfection with or without P.M OMV treatment. (F) Schematic diagram of miR-96-5p target site in the 3’UTR of Abca1 mRNA and its mutated sequence. (G) Relative firefly luciferase activity normalized to Renilla luciferase activity was shown. (H, I) Expression of Abca1 after NC and miR-96-5p mimic or inhibitor transfection by RT-qPCR and WB. (J, K) Expression of Abca1 after 0.15 μg/ml of P.M OMV treatment by RT-qPCR and WB. (L) Effect of miR-96-5p mimic on P.M OMVs mediated Abca1 and p-ERK expression. P.M, Proteus mirabilis. Data are from 3 independent experiments and given as mean ± SD. #p < 0.05; ##p < 0.01; ###p < 0.001 compared to NC mimic or M-CSF. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 compared to M-CSF + RANKL or NC mimic + P.M OMVs. ns, not significant.