Skip to main content
. 2022 Feb 28;8:90. doi: 10.1038/s41420-022-00894-w

Fig. 3. RIG-I is activated by ferrous myoglobin and interacts with caspase1.

Fig. 3

a–c qPCR and WB analyse RIG-I expression in macrophage treated with 200 μM ferrous myoglobin for 6 h, 12 h, and 24 h, respectively. c is a quantitative analysis of (b). d Representative confocal microscopy images of cells subjected to 200 μM ferrous myoglobin treatments and stained for nuclei (blue), anti-myoglobin (red), and RIG-I (green) to detect co-localization of myoglobin and RIG-I (Scale bars: 50 μm). e Representative cell light microscope images. Arrowheads indicate pyroptotic cells (Scale bars: 20 μm). f Schematic diagram of the molecular structure and possible interaction location between RIG-I and caspase1. g–h Co-IP assays detect the interaction between caspase1 and RIG-I in macrophages that treatment with 200 μM ferrous myoglobin for 12 h. i Representative confocal microscopy images of cells subjected to 200 μM ferrous myoglobin treatments and stained for nuclei (blue), RIG-I (green), and caspase1 (red) to detect co-localization of RIG-I and caspase1 (Scale bars: 50 μm). For statistical analysis, two-way ANOVA followed by Tukey’s method for multiple comparisons used in (a) and (c). Data is expressed as mean ± SD, n = 3 per group. **P < 0.01, ***P < 0.001.