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. 2022 Feb 28;8:90. doi: 10.1038/s41420-022-00894-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a–d qPCR analyses RIG-I, caspase1, GSDMD, IL-18 expression in control group (NC), siRIG-I group, ferrous myoglobin treatment group (Mb), and knocking down RIG-I then treatment with ferrous myoglobin group (siRIG-I + Mb). e WB analyses RIG-I, caspase1, cleaved-caspase1, GSDMD, N-GSDMD, cleaved-IL-1β expression in NC group, siRIG-I group, Mb group, and siRIG-I + Mb group. For statistical analysis, one-way ANOVA followed by Tukey’s method for multiple comparisons used in (a–d). Data are expressed as mean ± SD, n = 3 per group. *P < 0.05, **P < 0.01, ***P < 0.001.