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. 2022 Feb 28;8:90. doi: 10.1038/s41420-022-00894-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a–d qPCR analyses iNOS, CD86, TNF-α, IL-6 expression in NC, Mb, Mb + DMF, and DMF group. e, f WB and flow cytometry analyse iNOS expression in NC, Mb, Mb+DMF, and DMF group. g, h Representative confocal microscopy images of cells in NC, Mb, Mb + DMF, and DMF group stained for iNOS and CD86 (Scale bars: 20 μm). For statistical analysis, two-factor ANOVA followed by Tukey’s method of multiple comparisons used in (a–d). Data are expressed as mean ± SD, n = 3 per group. *P < 0.05, **P < 0.01, ***P < 0.001.