Fig. 4.

Detection of GvpC3GB and GvpC4GB binding with GVNPs. a Binding was carried out by incubation of GvpC3GB and GvpC4GB with a ratio of 1:5 (lanes: 1 and 2), 1:1 (lanes: 3 and 4), and 5:1 (lanes: 5 and 6) with GVNPs in 1 × PBS containing 3 M NaCl at room temperature for 1 h and then separated by centrifugation into the top (lanes: 2, 4, and 6), and subnatant (lanes: 1, 3, and 5) fractions for immunoblotting. Top fractions containing the GVNP complex were collected, mixed with 5 volumes of 1 × PBS containing 3 M NaCl, followed by b a second round of centrifugation. Immunoblotting was performed on the top (lanes: 2, 4, and 6) and subnatant (lanes: 1, 3, and 5) fractions