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. 2022 Mar 1;106(5-6):2043–2052. doi: 10.1007/s00253-022-11841-1

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© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022, corrected publication 2022

This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.

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Fig. 5 — Immunoplate assays of GvpC3GB fusion protein functionality on GVNP surface. a GVNPs and GvpC3GB were first incubated in 1 × PBS (spots 1 and 2) or 3 M NaCl (spots 3 and 4) at room temperature for 1 h followed by centrifugation at 300 × g for 30 min. The top phase containing GVNP::GvpC3GB was collected and further incubated with HRP-linked goat anti-rabbit IgG antibody followed by the centrifugal separation as above. b GVNPs were also incubated with HRP-linked goat anti-rabbit IgG antibody (spots 1 and 2) or with the GvpC3GB plus antibodies (lanes 3 and 4) in 3 M NaCl followed by centrifugal separation as above. An equal volume collected from GVNP-containing top (spots 2 and 4) and subnatant (spots 1 and 3) fractions were subject to chemiluminescence followed by densitometric quantification as shown in the graphs (blue: subnatant fraction, orange: top fraction)