Table 1.
Assay Abbreviations from the SS-Manuscript1
| assay | measure | effector | MOR | cells | indicator |
|---|---|---|---|---|---|
| Nb33 | Giα nanobody recruitment | Nb33-venus | flag-mMOR-NLuc | transient HEK293 | BRET |
| mGsi | mini-soluble Gi protein recruitment | NES-venus-mGsi | flag-mMOR-NLuc | transient HEK293 | BRET |
| Gai2 | association of Gβ with GRK3ct and dissociation from Gαi2 | Gαi2 venus-155–239-Gβ1 venus-1–155-Gγ2 |
flag-mMOR-NLuc | transient HEK293 | BRET |
| cAMP | cAMP biosensor | CAMYEL YFP-Epac-Rluc | flag-mMOR | transient HEK293 | BRET |
| βarr2+GRK2 | βarrestin2 recruitment with overexpressed GRK2 | βarr2-YFP (bovine) | flag-MOR-NLuc | transient HEK293 | BRET |