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. 2022 Feb 28;12:3295. doi: 10.1038/s41598-022-07305-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Establishment of Autologous CTOSs. (a) Formation of CTOSs from colorectal cancer tissues of three patients. Phase contrast images of organoid fraction: I, suspension culture; II, 3D culture. Scale bar = I, 100 μm; II, 40 μm. (b) Expression of EpCAM, CD45, and CEA in the CTOSs established from Case 1. Black-filled histograms represent the isotype-matched control. Gray lines highlight the staining results of EpCAM, CD45, and CEA.