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. 2022 Feb 28;12:3295. doi: 10.1038/s41598-022-07305-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cytotoxic activity of CTLs generated from iPSDCs-ivtRNA. CD8⁺ cytotoxic T cells were sorted using an autoMACS Pro Separator after three cycles of stimulation of autologous peripheral blood mononuclear cells, and their cytotoxic activity was tested using a 4-h ₅₁Cr release assay. The thick black line, dotted line, and thin black line represent the cytotoxic activity of CD8⁺ T cell induced with iPSDCs-CTOS ivtRNA, with iPSDCs-GFP ivtRNA, and with iPSDCs-CEA ivtRNA, respectively.