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. 2022 Feb 28;8:91. doi: 10.1038/s41420-022-00883-z

Fig. 3. Induction of EIF4EBP1 promoter activity by E2F6, ETS1, HIF-1A, and MYBL2.

Fig. 3

A Scheme of the luciferase reporter construct containing the EIF4EBP1 promoter, exon 1, and part of intron 1 (−661; +705), coupled to Firefly luciferase, with the indicated binding sites of transcription factor candidates. B–H HEK293-T cells were transfected with the −661; +705 EIF4EBP1 promoter reporter construct, together with increasing amounts of plasmids expressing either one of the indicated transcription factors and a vector expressing Renilla luciferase. Luciferase activities were detected using the Dual-Luciferase Reporter Assay. Firefly luciferase activity was normalized to Renilla luciferase activity and the ratio was normalized to the corresponding 0 ng condition. Data represent the mean of three independent replicates ± standard deviation (SD). Significance was calculated using an unpaired and one-tailed parametric t-test (*p < 0.05, **p < 0.01, ***p < 0.001 ****p < 0.0001). Below each diagram, a representative immunoblot analyzing overexpression of each of the indicated transcription factors is presented.