Table 2.

Main features of the different methods used at each lab participating to the external quality assurance total HIV-1 DNA quantification program.

Lab	Extraction method	Method to determine the total DNA concentration		Method to determine the HIV-1 DNA concentration		PCR master mix	Instrument	References
		Method	Target	Method	Target
lab01_qPCR	Abbott mSample Preparation System DNA Kit on Abbott m2000sp instrument; Abbott	qPCR	hTERT	qPCR	Integrase	Abbott RealTime HIV-1 Amplification Reagent Kit; Abbott	7500 Fast Dx RTPCR Instrument; Applied Biosystems	28,29
lab02_qPCR	QIAamp DNA Blood Mini Kit; Qiagen	qPCR	Albumin	qPCR	pol	PrecisionPLUS qPCR Master Mix; PrimerDesign	RotorGene Q; Qiagen	30
lab03_ddPCR	High pure PCR template preparation kit; Roche	ddPCR	Albumin	ddPCR	LTR	ddPCR™ Supermix for Probes (No dUTP); Biorad	QX200 Droplet Digital PCR System; BioRad	31
lab04_ddPCR	AllPrep DNA/RNA Mini Kit; Qiagen	ddPCR	rpp30	ddPCR	gag	ddPCR™ Supermix for Probes (No dUTP); Biorad	QX200 Droplet Digital PCR System; BioRad	32
lab05_qPCR	Qiacube, Qiamp DNA mini kit; Qiagen	Spectrophotometry		qPCR	pol	iTaqUniversal probes supermix; Biorad	ABI 7900; Applied Biosystem	33
lab06_qPCR	High pure PCR template preparation kit; Roche	qPCR	Beta Globin	qPCR	LTR	GoTaq probe qPCR master Mix; Promega	Eco Real-Time PCR system; Illumina	34
lab06_ddPCR	High pure PCR template preparation kit; Roche	ddPCR	Albumin	ddPCR	LTR	ddPCR™ Supermix for Probes (No dUTP); Biorad	QX200 Droplet Digital PCR System; BioRad	31
lab07_qPCR	QIAamp DNA Blood Mini Kit; Qiagen	Spectrophotometry		qPCR	LTR	Generic HIV DNA Cell kit; Biocentric	QuantStudio 5 Dx Real-Time PCR System; Thermo Fisher	35–37Commercial kit
lab08_qPCR	QIA Symphony DNA Blood Mini Kit; Qiagen	qPCR	hTERT	qPCR	LTR	GoTaq Probe qPCR Reaction Mix; Promega	LightCycler 2.0; Roche	34
lab08_ddPCR	QIA Symphony DNA Blood Mini Kit; Qiagen	ddPCR	Albumin	ddPCR	LTR	ddPCR™ Supermix for Probes (No dUTP); Biorad	QX200 Droplet Digital PCR System; BioRad	31,38
lab09_ddPCR	High pure PCR template preparation kit; Roche	ddPCR	Albumin	ddPCR	LTR	ddPCR™ Supermix for Probes (No dUTP); Biorad	QX200 Droplet Digital PCR System; BioRad	38
lab10_qPCR	QIA Symphony DNA Blood Mini Kit; Qiagen	qPCR	Beta Globin	qPCR	LTR	homemade	RotorGene Q; Qiagen	35
lab11_qPCR	QIAamp DNA Blood Mini Kit; Qiagen	qPCR	hTERT	qPCR	LTR	HIV-1 DNA Test PRO; Diatheva	ABI 7500; Applied Biosystem	39 Commercial Kit
lab12_qPCR	High pure viral nucleic acid kit; Roche	qPCR	Albumin	qPCR	LTR	PreMix ExTaq; Takara	LightCycler 96; Roche	27
lab12_ddPCR	High pure viral nucleic acid kit; Roche	ddPCR	Albumin	ddPCR	LTR	ddPCR™ Supermix for Probes (No dUTP); Biorad	QX200 Droplet Digital PCR System; BioRad	27

hTERT human Telomerase Reverse Transcriptase.